Article
A Noise Trimming and Positional Significance of Transposon Insertion System to Identify Essential Genes in Yersinia pestis.
Scientific reports - 6 Feb 2017
Yang Zheng Rong, Bullifent Helen L, Moore Karen, Paszkiewicz Konrad, Saint Richard J, Southern Stephanie J, Champion Olivia L, Senior Nicola J, Sarkar-Tyson Mitali, Oyston Petra C F, Atkins Timothy P, Titball Richard W
Abstract excerpt
Massively parallel sequencing technology coupled with saturation mutagenesis has provided new and global insights into gene functions and roles. At a simplistic level, the frequency of mutations within genes can indicate the degree of essentiality. However, this approach neglects to take account of the positional significance of mutations - the function of a gene is less likely to be disrupted by a mutation close...
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