Article
Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis.
Microbial pathogenesis - 1 Nov 2017
De Yanyan, Dong Congyue, Cao Yanyang, Wang Xiaolei, Yang Xiaowen, Wang Ning, Zhang Cunrui, Wang Zhen, Lyu Yanli, Wu Qingmin
Abstract excerpt
A transposon mutant library of B. melitensis NI including 32,640 transposon mutants was established. By sequencing the transposon insertion sites, 10,832 mutants were successfully defined for their insertion sites. Analysis of the mutants with defined transposon insertion sites (DTIS) indicated that the insertions were well spread through the two genomes. In addition, 948 genes with no detectable transposon...
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