Crystal Structure of Green Fluorescent Protein Clover and Design of Clover-Based Redox Sensors.

Abstract excerpt

We have determined the crystal structure of Clover, one of the brightest fluorescent proteins, and found that its T203H/S65G mutations relative to wild-type GFP lock the critical E222 side chain in a fixed configuration that mimics the major conformer of that in EGFP. The resulting equilibrium shift to the predominantly deprotonated chromophore increases the extinction coefficient (EC), opposes photoactivation,...

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• Crystallography, X-Ray
• Green Fluorescent Proteins
• Medicago
• Models, Molecular
• Mutation