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Article

Structure and mechanism of the photoactivatable green fluorescent protein.

Journal of the American Chemical Society - 1 Apr 2009

Henderson J Nathan, Gepshtein Rinat, Heenan Josef R, Kallio Karen, Huppert Dan, Remington S James

Abstract excerpt

Crystal structures of the photoactivatable green fluorescent protein T203H variant (PA-GFP) have been solved in the native and photoactivated states, which under 488 nm illumination are dark and brightly fluorescent, respectively. We demonstrate that photoactivation of PA-GFP is the result of a UV-induced decarboxylation of the Glu222 side chain that shifts the chromophore equilibrium to the anionic form. Coupled...

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PMID: 19278226
DOI: 10.1021/ja808851n

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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