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Article

Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.

Proceedings of the National Academy of Sciences of the United States of America - 14 Oct 2003

Barondeau David P, Putnam Christopher D, Kassmann Carey J, Tainer John A, Getzoff Elizabeth D

Abstract excerpt

Green fluorescent protein has revolutionized cell labeling and molecular tagging, yet the driving force and mechanism for its spontaneous fluorophore synthesis are not established. Here we discover mutations that substantially slow the rate but not the yield of this posttranslational modification, determine structures of the trapped precyclization intermediate and oxidized postcyclization states, and identify...

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Biophysical Phenomena
Biophysics
Crystallography, X-Ray
Energy Metabolism
Green Fluorescent Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Luminescent Proteins

Oxidation-Reduction

Protein Processing, Post-Translational

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Identifiers

PMID: 14523232
DOI: 10.1073/pnas.2133463100
PMCID: PMC218721

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