Article
Designed active-site library reveals thousands of functional GFP variants.
Nature communications - 20 May 2023
Weinstein Jonathan Yaacov, Martí-Gómez Carlos, Lipsh-Sokolik Rosalie, Hoch Shlomo Yakir, Liebermann Demian, Nevo Reinat, Weissman Haim, Petrovich-Kopitman Ekaterina, Margulies David, Ivankov Dmitry, McCandlish David M, Fleishman Sarel J
Abstract excerpt
Mutations in a protein active site can lead to dramatic and useful changes in protein activity. The active site, however, is sensitive to mutations due to a high density of molecular interactions, substantially reducing the likelihood of obtaining functional multipoint mutants. We introduce an atomistic and machine-learning-based approach, called high-throughput Functional Libraries (htFuncLib), that designs a...
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