Article

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

2016-02-18

Abstract excerpt

Cas9 endonuclease can be targeted to genomic sequences by varying the sequence of the single guide RNA (sgRNA). The activity of these Cas9-sgRNA combinations varies widely at different genomic loci and in different cell types. Thus, disrupting genes in polyploid cell lines, or using inefficient sgRNAs, can require extensive downstream screening to identify homozygous clones. We have found that linear, non-homologo...

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Literature Corpus work: 62c13cfc-5d00-559c-b309-631611b2eab4
DOI: 10.1101/040212

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