Article
Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides.
Scientific reports - 9 Sept 2016
Bialk Pawel, Sansbury Brett, Rivera-Torres Natalia, Bloh Kevin, Man Dula, Kmiec Eric B
Abstract excerpt
The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system. While the mechanism of action of combinatorial gene editing remains to be elucidated, the regulatory circuitry of nucleotide exchange executed by oligonucleotides alone has been largely defined. The presence of the appropriate CRISPR/Cas9 system leads to an enhancement in the...
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