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Article

Stable gene targeting in human cells using single-strand oligonucleotides with modified bases.

PloS one - 1 Jan 2012

Rios Xavier, Briggs Adrian W, Christodoulou Danos, Gorham Josh M, Seidman Jonathan G, Church George M

Abstract excerpt

Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, both by enhancing our ability to test how individual variants such as single nucleotide polymorphisms (SNPs) are related to specific phenotypes, and potentially allowing simultaneous...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 22615794
DOI: 10.1371/journal.pone.0036697
PMCID: PMC3351460

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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