Article
High-Resolution Melting (HRM) Genotyping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Kim Nayoung, Kwon Ji-Su, Kang Won-Hee, Yeom Seon-In
Abstract excerpt
High-resolution melting (HRM) analysis is a simple, fast, and inexpensive real-time polymerase chain reaction (PCR)-based method used to identify genetic variation between populations and detect single-nucleotide polymorphisms (SNPs) in nucleic acid sequences. HRM is a powerful technique that detects the differences between SNP allele melting temperatures by using a fluorescent dye inserted into the duplex...
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