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Article

DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo.

Nature methods - 1 Jan 2017

Zubradt Meghan, Gupta Paromita, Persad Sitara, Lambowitz Alan M, Weissman Jonathan S, Rouskin Silvi

Abstract excerpt

Coupling of structure-specific in vivo chemical modification to next-generation sequencing is transforming RNA secondary structure studies in living cells. The dominant strategy for detecting in vivo chemical modifications uses reverse transcriptase truncation products, which introduce biases and necessitate population-average assessments of RNA structure. Here we present dimethyl sulfate (DMS) mutational...

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Identifiers

PMID: 27819661
DOI: 10.1038/nmeth.4057
PMCID: PMC5508988

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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