Article
Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Song Shikui, Su Zhengding
Abstract excerpt
Targeted mutagenesis by homologous recombination (TMHR) is an efficient allelic exchange mutagenesis for bacterial genome engineering in synthetic biology. Unlike other allelic exchange methods, TMHR does not require a heterologous recombinase to insert or excise a selectable marker from the genome. In contrast, positive and negative selection is achieved solely by suicide vector-encoded functional and host cell...
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