Article
Co-CRISPR: A valuable toolkit for mutation enrichment in the gene editing of Spodoptera frugiperda.
Insect science - 1 Jun 2023
Han Wei-Kang, Tang Feng-Xian, Gao Hao-Li, Wang Yan, Yu Na, Jiang Jian-Jun, Liu Ze-Wen
Abstract excerpt
The CRISPR/Cas9 system has been successfully applied in dozens of diverse species; although the screening of successful CRISPR/Cas9 editing events remains particularly laborious, especially for those that occur at relatively low frequency. Recently, a co-CRISPR strategy was proved to enrich the desired CRISPR events. Here, the co-CRISPR strategy was developed in the Fall armyworm, Spodoptera frugiperda, with...
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