Article
Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion.
G3 (Bethesda, Md.) - 13 Oct 2016
Ge Daniel Tianfang, Tipping Cindy, Brodsky Michael H, Zamore Phillip D
Abstract excerpt
Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CRISPR-like single-guide RNA, catalyzes a double-stranded DNA break at a specific genomic site; subsequent repair by end joining can introduce mutagenic insertions or...
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