Article
ovoD Co-selection: A Method for Enriching CRISPR/Cas9-Edited Alleles in Drosophila.
G3 (Bethesda, Md.) - 31 Jul 2018
Ewen-Campen Ben, Perrimon Norbert
Abstract excerpt
Screening for successful CRISPR/Cas9 editing events remains a time consuming technical bottleneck in the field of Drosophila genome editing. This step can be particularly laborious for events that do not cause a visible phenotype, or those which occur at relatively low frequency. A promising strategy to enrich for desired CRISPR events is to co-select for an independent CRISPR event that produces an easily...
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