Analyzing structure-function relationships of artificial and cancer-associated PARP1 variants by reconstituting TALEN-generated HeLa PARP1 knock-out cells.

doi:10.1093/nar/gkw859

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Article

Analyzing structure-function relationships of artificial and cancer-associated PARP1 variants by reconstituting TALEN-generated HeLa PARP1 knock-out cells.

Nucleic acids research - 1 Dec 2016

Rank Lisa, Veith Sebastian, Gwosch Eva C, Demgenski Janine, Ganz Magdalena, Jongmans Marjolijn C, Vogel Christopher, Fischbach Arthur, Buerger Stefanie, Fischer Jan M F, Zubel Tabea, Stier Anna, Renner Christina, Schmalz Michael, Beneke Sascha, Groettrup Marcus, Kuiper Roland P, Bürkle Alexander, Ferrando-May Elisa, Mangerich Aswin

Abstract excerpt

Genotoxic stress activates PARP1, resulting in the post-translational modification of proteins with poly(ADP-ribose) (PAR). We genetically deleted PARP1 in one of the most widely used human cell systems, i.e. HeLa cells, via TALEN-mediated gene targeting. After comprehensive characterization of these cells during genotoxic stress, we analyzed structure-function relationships of PARP1 by reconstituting PARP1 KO...

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PMID: 27694308
DOI: 10.1093/nar/gkw859
PMCID: PMC5137445

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PARP1

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