Article
Kinetics of poly(ADP-ribosyl)ation, but not PARP1 itself, determines the cell fate in response to DNA damage in vitro and in vivo.
Nucleic acids research - 2 Nov 2017
Schuhwerk Harald, Bruhn Christopher, Siniuk Kanstantsin, Min Wookee, Erener Suheda, Grigaravicius Paulius, Krüger Annika, Ferrari Elena, Zubel Tabea, Lazaro David, Monajembashi Shamci, Kiesow Kirstin, Kroll Torsten, Bürkle Alexander, Mangerich Aswin, Hottiger Michael, Wang Zhao-Qi
Abstract excerpt
One of the fastest cellular responses to genotoxic stress is the formation of poly(ADP-ribose) polymers (PAR) by poly(ADP-ribose)polymerase 1 (PARP1, or ARTD1). PARP1 and its enzymatic product PAR regulate diverse biological processes, such as DNA repair, chromatin remodeling, transcription and cell death. However, the inter-dependent function of the PARP1 protein and its enzymatic activity clouds the mechanism...
Topics
- Animals
- Catalytic Domain
- Cells, Cultured
- DNA Damage
- DNA Repair
- DNA Replication
- Kinetics
- Mice
- Mice, 129 Strain
- Mice, Transgenic