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Article

An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease.

Genome biology - 15 Sept 2016

Xu Shu, Cao Shasha, Zou Bingjie, Yue Yunyun, Gu Chun, Chen Xin, Wang Pei, Dong Xiaohua, Xiang Zheng, Li Kai, Zhu Minsheng, Zhao Qingshun, Zhou Guohua

Abstract excerpt

Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3' flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands. The SGN recognizes the target DNA on the basis of the 3' flap structure formed between the target...

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Share to Deoxyribonucleases, Type II Site-Specific
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27634179
DOI: 10.1186/s13059-016-1038-5
PMCID: PMC5025552

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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