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Article

The flexible loop of human FEN1 endonuclease is required for flap cleavage during DNA replication and repair.

The EMBO journal - 1 Nov 2002

Storici Francesca, Henneke Ghislaine, Ferrari Elena, Gordenin Dmitry A, Hübscher Ulrich, Resnick Michael A

Abstract excerpt

The conserved, structure-specific flap endonuclease FEN1 cleaves 5' DNA flaps that arise during replication or repair. To address in vivo mechanisms of flap cleavage, we developed a screen for human FEN1 mutants that are toxic when expressed in yeast. Two targets were revealed: the flexible loop domain and the catalytic site. Toxic mutants caused G(2) arrest and cell death and were unable to repair methyl...

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Source

Open in PubMed Open full text

Identifiers

PMID: 12411510
DOI: 10.1093/emboj/cdf587
PMCID: PMC131084

Genes

FEN1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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The flexible loop of human FEN1 endonuclease is required for flap cleavage during DNA replication and repair.PMID 12411510Read ↗

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XPF mediates 3’ flap processing for FEN1-independent Okazaki fragment maturationSemantic proximity Read ↗

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