Article
The flexible loop of human FEN1 endonuclease is required for flap cleavage during DNA replication and repair.
The EMBO journal - 1 Nov 2002
Storici Francesca, Henneke Ghislaine, Ferrari Elena, Gordenin Dmitry A, Hübscher Ulrich, Resnick Michael A
Abstract excerpt
The conserved, structure-specific flap endonuclease FEN1 cleaves 5' DNA flaps that arise during replication or repair. To address in vivo mechanisms of flap cleavage, we developed a screen for human FEN1 mutants that are toxic when expressed in yeast. Two targets were revealed: the flexible loop domain and the catalytic site. Toxic mutants caused G(2) arrest and cell death and were unable to repair methyl...
Topics
- Base Sequence
- Catalytic Domain
- DNA
- DNA Repair
- DNA Replication
- Endodeoxyribonucleases
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Flap Endonucleases
- Humans
- Hydrolysis