Article
SHAPE reveals transcript-wide interactions, complex structural domains, and protein interactions across the Xist lncRNA in living cells.
Proceedings of the National Academy of Sciences of the United States of America - 13 Sept 2016
Smola Matthew J, Christy Thomas W, Inoue Kaoru, Nicholson Cindo O, Friedersdorf Matthew, Keene Jack D, Lee David M, Calabrese J Mauro, Weeks Kevin M
Abstract excerpt
The 18-kb Xist long noncoding RNA (lncRNA) is essential for X-chromosome inactivation during female eutherian mammalian development. Global structural architecture, cell-induced conformational changes, and protein-RNA interactions within Xist are poorly understood. We used selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) to examine these features of Xist at...
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