Article
Molecular Determinants of Mutant Phenotypes, Inferred from Saturation Mutagenesis Data.
Molecular biology and evolution - 1 Nov 2016
Tripathi Arti, Gupta Kritika, Khare Shruti, Jain Pankaj C, Patel Siddharth, Kumar Prasanth, Pulianmackal Ajai J, Aghera Nilesh, Varadarajan Raghavan
Abstract excerpt
Understanding how mutations affect protein activity and organismal fitness is a major challenge. We used saturation mutagenesis combined with deep sequencing to determine mutational sensitivity scores for 1,664 single-site mutants of the 101 residue Escherichia coli cytotoxin, CcdB at seven different expression levels. Active-site residues could be distinguished from buried ones, based on their differential...
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