Article
Molecular bases for strong phenotypic effects of single synonymous codon substitutions in the E. coli ccdB toxin gene.
BMC genomics - 4 Dec 2023
Bajaj Priyanka, Bhasin Munmun, Varadarajan Raghavan
Abstract excerpt
BACKGROUND: Single synonymous codon mutations typically have only minor or no effects on gene function. Here, we estimate the effects on cell growth of ~ 200 single synonymous codon mutations in an operonic context by mutating almost all positions of ccdB, the 101-residue long cytotoxin of the ccdAB Toxin-Antitoxin (TA) operon to most degenerate codons. Phenotypes were assayed by transforming the mutant library...
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