Article
Structural correlates of the temperature sensitive phenotype derived from saturation mutagenesis studies of CcdB.
Biochemistry - 9 Dec 2008
Bajaj Kanika, Dewan Pooja C, Chakrabarti Purbani, Goswami Devrishi, Barua Bipasha, Baliga Chetana, Varadarajan Raghavan
Abstract excerpt
Temperature sensitive (ts) mutants are widely used to reversibly modulate protein function in vivo and to understand functions of essential genes. Despite this, little is known about the protein structural features and mechanisms responsible for generating a ts phenotype. Also, such mutants are often difficult to isolate, limiting their use. In this study, a library consisting of 75% of all possible single-site...
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