Article
Design of temperature-sensitive mutants solely from amino acid sequence.
Proceedings of the National Academy of Sciences of the United States of America - 25 May 2004
Chakshusmathi Ghadiyaram, Mondal Kajari, Lakshmi G Santosh, Singh Guramrit, Roy Ankita, Ch Ravindra Babu, Madhusudhanan S, Varadarajan Raghavan
Abstract excerpt
Temperature-sensitive (Ts) mutants are a powerful tool with which to study gene function in vivo. Ts mutants are typically generated by random mutagenesis followed by laborious screening procedures. By using the Escherichia coli cytotoxin CcdB as a model system, simple procedures for generating Ts mutants at high frequency through site-directed mutagenesis were developed. Putative buried, hydrophobic residues are...
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