Article
The High Mutational Sensitivity of ccdA Antitoxin Is Linked to Codon Optimality.
Molecular biology and evolution - 7 Oct 2022
Chandra Soumyanetra, Gupta Kritika, Khare Shruti, Kohli Pehu, Asok Aparna, Mohan Sonali Vishwa, Gowda Harsha, Varadarajan Raghavan
Abstract excerpt
Deep mutational scanning studies suggest that synonymous mutations are typically silent and that most exposed, nonactive-site residues are tolerant to mutations. Here, we show that the ccdA antitoxin component of the Escherichia coli ccdAB toxin-antitoxin system is unusually sensitive to mutations when studied in the operonic context. A large fraction (∼80%) of single-codon mutations, including many synonymous...
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