Article
Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting.
Protein engineering, design & selection : PEDS - 1 Sept 2016
Obexer Richard, Pott Moritz, Zeymer Cathleen, Griffiths Andrew D, Hilvert Donald
Abstract excerpt
De novo biocatalysts with non-natural functionality are accessible by computational enzyme design. The catalytic activities obtained for the initial designs are usually low, but can be optimized significantly by directed evolution. Nevertheless, rate accelerations approaching the level of natural enzymes can only be achieved over many rounds of tedious and time-consuming laboratory evolution. In this work, we...
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