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Article

Optimization of Enzyme Mechanism along the Evolutionary Trajectory of a Computationally Designed (Retro-)Aldolase.

Journal of the American Chemical Society - 13 Sept 2017

Zeymer Cathleen, Zschoche Reinhard, Hilvert Donald

Abstract excerpt

De novo biocatalysts have been successfully generated by computational design and subsequent experimental optimization. Here, we examined the evolutionary history of the computationally designed (retro-)aldolase RA95. The modest activity of the starting enzyme was previously improved 105-fold over many rounds of mutagenesis and screening to afford a proficient biocatalyst for enantioselective cleavage and...

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PMID: 28783336
DOI: 10.1021/jacs.7b05796

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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