Article
High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets.
ACS synthetic biology - 20 Dec 2019
van Loo Bert, Heberlein Magdalena, Mair Philip, Zinchenko Anastasia, Schüürmann Jan, Eenink Bernard D G, Holstein Josephin M, Dilkaute Carina, Jose Joachim, Hollfelder Florian, Bornberg-Bauer Erich
Abstract excerpt
Directed evolution of enzymes toward improved catalytic performance has become a powerful tool in protein engineering. To be effective, a directed evolution campaign requires the use of high-throughput screening. In this study we describe the development of an ultra high-throughput lysis-free procedure to screen for improved sulfatase activity by combining microdroplet-based single-variant activity sorting with...
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