Article
Vector redesign and in-droplet cell-growth improves enrichment and recovery in live Escherichia coli.
Microbial biotechnology - 1 Nov 2022
Eenink Bernard D G, Kaminski Tomasz S, Bornberg-Bauer Erich, Jose Joachim, Hollfelder Florian, van Loo Bert
Abstract excerpt
Directed evolution (DE) is a widely used method for improving the function of biomolecules via multiple rounds of mutation and selection. Microfluidic droplets have emerged as an important means to screen the large libraries needed for DE, but this approach was so far partially limited by the need to lyse cells, recover DNA, and retransform into cells for the next round, necessitating the use of a high-copy...
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