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Article

Exploring sequence space in search of functional enzymes using microfluidic droplets.

Current opinion in chemical biology - 1 Apr 2017

Mair Philip, Gielen Fabrice, Hollfelder Florian

Abstract excerpt

Screening of enzyme mutants in monodisperse picoliter compartments, generated at kilohertz speed in microfluidic devices, is coming of age. After a decade of proof-of-principle experiments, workflows have emerged that combine existing microfluidic modules to assay reaction progress quantitatively and yield improved enzymes. Recent examples of the screening of libraries of randomised proteins and from metagenomic...

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PMID: 28390246
DOI: 10.1016/j.cbpa.2017.02.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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