Article

Efficient cell-free evolution of RNA polymerases by droplet microfluidics

2026-02-23

Abstract excerpt

Directed evolution enables the rapid creation of biomolecules with new functions, yet linking genotype and phenotype often requires laborious cell-based workflows. Here we integrate a boosted in vitro transcription–translation system capable of protein expression from single-copy DNA with ultrahigh-throughput fluorescence-activated droplet sorting, developing a simple one-day workflow for efficient protein evolut...

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Literature Corpus work: adff9a53-ca35-5ab9-9eae-5a2a9d86da68
DOI: 10.64898/2026.02.23.707346

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