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Article

Dissecting enzyme function with microfluidic-based deep mutational scanning.

Proceedings of the National Academy of Sciences of the United States of America - 9 Jun 2015

Romero Philip A, Tran Tuan M, Abate Adam R

Abstract excerpt

Natural enzymes are incredibly proficient catalysts, but engineering them to have new or improved functions is challenging due to the complexity of how an enzyme's sequence relates to its biochemical properties. Here, we present an ultrahigh-throughput method for mapping enzyme sequence-function relationships that combines droplet microfluidic screening with next-generation DNA sequencing. We apply our method to...

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Identifiers

PMID: 26040002
DOI: 10.1073/pnas.1422285112
PMCID: PMC4466731

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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