μIVC-Seq: A Method for Ultrahigh-Throughput Development and Functional Characterization of Small RNAs.

doi:10.1007/978-1-0716-1386-3_17

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Article

μIVC-Seq: A Method for Ultrahigh-Throughput Development and Functional Characterization of Small RNAs.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Bouhedda Farah, Cubi Roger, Baudrey Stéphanie, Ryckelynck Michael

Abstract excerpt

For a long time, artificial RNAs have been developed by in vitro selection methodologies like Systematic Evolution of Ligands by EXponential enrichment (SELEX). Yet, even though this technology is extremely powerful to isolate specific and high-affinity binders, it is less suited for the isolation of RNAs optimized for more complex functions such as fluorescence emission or multiple-turnover catalysis. Whereas...

Topics

Computational Biology
Gene Library
High-Throughput Nucleotide Sequencing
Microfluidics
Miniaturization
Mutation
RNA, Messenger
Sequence Analysis, RNA

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PMID: 33792882
DOI: 10.1007/978-1-0716-1386-3_17

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