Article
Directed enzyme evolution: beyond the low-hanging fruit.
Current opinion in structural biology - 1 Aug 2012
Goldsmith Moshe, Tawfik Dan S
Abstract excerpt
The field of directed evolution has progressed to the point where it is feasible to engineer enzymes for unnatural substrates and reactions with catalytic efficiencies and regio-specificity or stereo-specificity that rival those of natural enzymes. Here, we describe the conceptual and methodological advances that have enabled this progress. We address methodologies based on small libraries enriched with improved...
Topics
- Directed Molecular Evolution
- Enzyme Assays
- Enzymes
- Gene Library
- Humans
- Mutation
- Protein Engineering