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Article

Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene.

Science China. Life sciences - 1 Feb 2017

Chen Yuting, Lu Wenqing, Gao Na, Long Yi, Shao Yanjiao, Liu Meizhen, Chen Huaqing, Ye Shixin, Ma Xueyun, Liu Mingyao, Li Dali

Abstract excerpt

The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases (TALENs) technology to generate Leptin receptor (Lepr) knockout rats on the Sprague Dawley (SD) genetic background. Through direct injection of in vitro transcribed mRNA of TALEN pairs into SD rat zygotes, somatic...

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Identifiers

PMID: 27225180
DOI: 10.1007/s11427-016-5049-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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