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Article

A sensitive mutation screening method supporting cell line development for biotherapeutics.

Analytical biochemistry - 15 Jul 2016

Valisheva Ildana, Harris Reed J, Zhu-Shimoni Judith

Abstract excerpt

Random genetic mutations, which can occur during cell line development, can lead to sequence variants that comprise pharmaceutical product quality generated by recombinant technology. Mutation screening can minimize the probability of selecting clones harboring sequence variants. Here we report a polymerase chain reaction (PCR)-based mutation screening approach using high-resolution melting (HRM) analysis...

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Identifiers

PMID: 27108188
DOI: 10.1016/j.ab.2016.04.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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