Article
DMSO Increases Mutation Scanning Detection Sensitivity of High-Resolution Melting in Clinical Samples.
Clinical chemistry - 1 Nov 2015
Song Chen, Castellanos-Rizaldos Elena, Bejar Rafael, Ebert Benjamin L, Makrigiorgos G Mike
Abstract excerpt
BACKGROUND: Mutation scanning provides the simplest, lowest-cost method for identifying DNA variations on single PCR amplicons, and it may be performed before sequencing to avoid screening of noninformative wild-type samples. High-resolution melting (HRM) is the most commonly used method for mutation scanning. With PCR-HRM, however, mutations less abundant than approximately 3%-10% that can still be clinically...
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