Article
A one-step prescreening for point mutations and large rearrangement in BRCA1 and BRCA2 genes using quantitative polymerase chain reaction and high-resolution melting curve analysis.
Genetic testing and molecular biomarkers - 1 Oct 2010
Coulet Florence, Pires Filipe, Rouleau Etienne, Lefol Cedrick, Martin Sabrina, Colas Chrystelle, Cohen-Haguenauer Odile, Giurgea Irina, Fajac Anne, Noguès Catherine, Demange Liliane, Hardouin Agnès, Lidereau Rosette, Soubrier Florent
Abstract excerpt
High-resolution melting (HRM) of DNA is a versatile method for mutation scanning that monitors the fluorescence of double-strand DNA with saturating dye. Performing HRM on a real-time thermocycler enables semiquantitative analysis (quantitative polymerase chain reaction, qPCR) to be associated to...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.