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Article

Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection.

BMC molecular biology - 2 Apr 2016

Estep Jason A, Sternburg Erin L, Sanchez Gissell A, Karginov Fedor V

Abstract excerpt

BACKGROUND: Targeted genomic editing using the CRISPR/Cas9 methodology has opened exciting new avenues in probing gene function in virtually any model system, including cultured mammalian cells. Depending on the desired mutation, several experimental options exist in the isolation of clonal lines, such as selection with introduced markers, or screening by PCR amplification of genomic DNA. However, streamlined...

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Identifiers

PMID: 27038923
DOI: 10.1186/s12867-016-0061-0
PMCID: PMC4818936

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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