Article
Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection.
BMC molecular biology - 2 Apr 2016
Estep Jason A, Sternburg Erin L, Sanchez Gissell A, Karginov Fedor V
Abstract excerpt
BACKGROUND: Targeted genomic editing using the CRISPR/Cas9 methodology has opened exciting new avenues in probing gene function in virtually any model system, including cultured mammalian cells. Depending on the desired mutation, several experimental options exist in the isolation of clonal lines...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Cell Line
- ELAV-Like Protein 1
- Gene Knockout Techniques
- Gene Targeting
- Genotype
- Humans
- Immunoblotting
- Molecular Sequence Data
- Mutation
- RNA, Guide, CRISPR-Cas Systems
- Sequence Alignment