Article
A mutant RNA polymerase that forms unusual open promoter complexes.
Proceedings of the National Academy of Sciences of the United States of America - 9 Dec 1997
Severinov K, Darst S A
Abstract excerpt
We describe a mutant Escherichia coli RNA polymerase (RNAP) that forms stable open promoter complexes even at -20 degrees C but with a shortened melted region that extends downstream to only position -7. In the presence of initiating transcription substrates, the mutant RNAP undergoes a temperatu...
Topics
- Base Sequence
- Binding Sites
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Protein Conformation
- Sequence Deletion
- Temperature
- Thermodynamics
- Transcription, Genetic