Article
Whole-genome mutational burden analysis of three pluripotency induction methods.
Nature communications - 19 Feb 2016
Bhutani Kunal, Nazor Kristopher L, Williams Roy, Tran Ha, Dai Heng, Džakula Željko, Cho Edward H, Pang Andy W C, Rao Mahendra, Cao Han, Schork Nicholas J, Loring Jeanne F
Abstract excerpt
There is concern that the stresses of inducing pluripotency may lead to deleterious DNA mutations in induced pluripotent stem cell (iPSC) lines, which would compromise their use for cell therapies. Here we report comparative genomic analysis of nine isogenic iPSC lines generated using three reprogramming methods: integrating retroviral vectors, non-integrating Sendai virus and synthetic mRNAs. We used...
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