Article
Background mutations in parental cells account for most of the genetic heterogeneity of induced pluripotent stem cells.
Cell stem cell - 4 May 2012
Young Margaret A, Larson David E, Sun Chiao-Wang, George Daniel R, Ding Li, Miller Christopher A, Lin Ling, Pawlik Kevin M, Chen Ken, Fan Xian, Schmidt Heather, Kalicki-Veizer Joelle, Cook Lisa L, Swift Gary W, Demeter Ryan T, Wendl Michael C, Sands Mark S, Mardis Elaine R, Wilson Richard K, Townes Tim M, Ley Timothy J
Abstract excerpt
To assess the genetic consequences of induced pluripotent stem cell (iPSC) reprogramming, we sequenced the genomes of ten murine iPSC clones derived from three independent reprogramming experiments, and compared them to their parental cell genomes. We detected hundreds of single nucleotide variants (SNVs) in every clone, with an average of 11 in coding regions. In two experiments, all SNVs were unique for each...
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