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Article

Chicken scFvs with an Artificial Cysteine for Site-Directed Conjugation.

PloS one - 1 Jan 2016

Yoon Aerin, Shin Jung Won, Kim Soohyun, Kim Hyori, Chung Junho

Abstract excerpt

For the site-directed conjugation of chemicals and radioisotopes to the chicken-derived single-chain variable fragment (scFv), we investigated amino acid residues replaceable with cysteine. By replacing each amino acid of the 157 chicken variable region framework residues (FR, 82 residues on VH and 75 on VL) with cysteine, 157 artificial cysteine mutants were generated and characterized. At least 27 residues on...

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Identifiers

PMID: 26764487
DOI: 10.1371/journal.pone.0146907
PMCID: PMC4713166

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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