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Article

Site-specific conjugation on serine right-arrow cysteine variant monoclonal antibodies.

The Journal of biological chemistry - 29 Sept 2000

Stimmel J B, Merrill B M, Kuyper L F, Moxham C P, Hutchins J T, Fling M E, Kull F C

Abstract excerpt

We have engineered a cysteine residue at position 442 (EU/OU numbering) in the third constant domain (C(H)3) of the heavy chain of several IgGs with different specificities, isoforms, and variants with the intent to introduce a site for chemical conjugation. The variants were expressed in NS0 mouse myeloma cells, where monomeric IgG is the major form and formation of aggregate was minimal. Monomeric IgG contained...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 10880507
DOI: 10.1074/jbc.M001672200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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