Article
Sequential protection-modification method for selective sulfhydryl group derivatization in proteins having more than one cysteine.
Protein engineering - 1 Jul 1990
Planas A, Kirsch J F
Abstract excerpt
The method of Smith and Hartman [J. Biol. Chem., 263, 4921-4925 (1988)] for introducing the non-natural lysine analog, S-(2-aminoethyl)cysteine, into specific sites in proteins by alkylation of a genetically introduced cysteine with 2-bromoethylamine has been generalized to be applicable to proteins containing one or more endogenous cysteines. The target cysteine residue introduced at the active site of aspartate...
Topics
- Alkylating Agents
- Aspartate Carbamoyltransferase
- Chemical Phenomena
- Chemistry
- Cysteine
- Kinetics
- Mutation
- Protein Conformation
- Protein Denaturation
- Protein Engineering
- Sulfhydryl Compounds