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Article

Sequential protection-modification method for selective sulfhydryl group derivatization in proteins having more than one cysteine.

Protein engineering - 1 Jul 1990

Planas A, Kirsch J F

Abstract excerpt

The method of Smith and Hartman [J. Biol. Chem., 263, 4921-4925 (1988)] for introducing the non-natural lysine analog, S-(2-aminoethyl)cysteine, into specific sites in proteins by alkylation of a genetically introduced cysteine with 2-bromoethylamine has been generalized to be applicable to proteins containing one or more endogenous cysteines. The target cysteine residue introduced at the active site of aspartate...

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Identifiers

PMID: 2217135
DOI: 10.1093/protein/3.7.625

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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