Article
Quantitative Detection and Resolution of BRAF V600 Status in Colorectal Cancer Using Droplet Digital PCR and a Novel Wild-Type Negative Assay.
The Journal of molecular diagnostics : JMD - 1 Mar 2016
Bidshahri Roza, Attali Dean, Fakhfakh Kareem, McNeil Kelly, Karsan Aly, Won Jennifer R, Wolber Robert, Bryan Jennifer, Hughesman Curtis, Haynes Charles
Abstract excerpt
A need exists for robust and cost-effective assays to detect a single or small set of actionable point mutations, or a complete set of clinically informative mutant alleles. Knowledge of these mutations can be used to alert the clinician to a rare mutation that might necessitate more aggressive c...
Topics
- Alleles
- Cell Line, Tumor
- Colorectal Neoplasms
- DNA Mutational Analysis
- DNA Probes
- Humans
- Limit of Detection
- Paraffin Embedding
- Plasmids
- Point Mutation
- Polymerase Chain Reaction
- Proto-Oncogene Mas
- Proto-Oncogene Proteins B-raf