Article
Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines.
Journal of visualized experiments : JoVE - 8 Oct 2015
Rajasekaran Nirmal, Oh Myung Ryurl, Kim Sung-Su, Kim Si Eun, Kim Young Deug, Choi Hyun-Jeung, Byun Bohyun, Shin Young Kee
Abstract excerpt
ddPCR is a highly sensitive PCR method that utilizes a water-oil emulsion system. Using a droplet generator, an extracted nucleic acid sample is partitioned into ~20,000 nano-sized, water-in-oil droplets, and PCR amplification occurs in individual droplets. The ddPCR approach is in identifying sequence mutations, copy number alterations, and select structural rearrangements involving targeted genes. Here, we...
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