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Article

Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach.

PloS one - 1 Jan 2015

Pender Alexandra, Garcia-Murillas Isaac, Rana Sareena, Cutts Rosalind J, Kelly Gavin, Fenwick Kerry, Kozarewa Iwanka, Gonzalez de Castro David, Bhosle Jaishree, O'Brien Mary, Turner Nicholas C, Popat Sanjay, Downward Julian

Abstract excerpt

Droplet digital PCR (ddPCR) can be used to detect low frequency mutations in oncogene-driven lung cancer. The range of KRAS point mutations observed in NSCLC necessitates a multiplex approach to efficient mutation detection in circulating DNA. Here we report the design and optimisation of three discriminatory ddPCR multiplex assays investigating nine different KRAS mutations using PrimePCR™ ddPCR™ Mutation Assays...

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Identifiers

PMID: 26413866
DOI: 10.1371/journal.pone.0139074
PMCID: PMC4586384

Genes

KRAS

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