Article
Clinical validation and implementation of droplet digital PCR for the detection of BRAF mutations from cell-free DNA.
Pathology - 1 Oct 2022
Arnolda Rainier, Howlett Kerryn, Chan Timmy, Raleigh Jeanette, Hatzimihalis Athena, Bell Anthony, Fellowes Andrew, Sandhu Shahneen, McArthur Grant A, Fox Stephen B, Dawson Sarah-Jane, Hewitt Chelsee, Jones Kate, Wong Stephen Q
Abstract excerpt
Droplet digital PCR (ddPCR) has been demonstrated in many research studies to be a sensitive method in the analysis of circulating tumour DNA (ctDNA) for identifying mutations and tracking disease. The transition of ddPCR into the diagnostic setting requires a number of critical steps including the assessment of accuracy and precision and ultimately implementation into clinical use. Here we present the clinical...
Topics
- Cell-Free Nucleic Acids
- Circulating Tumor DNA
- DNA Mutational Analysis
- Formaldehyde
- Humans
- Mutation
- Polymerase Chain Reaction
- Proto-Oncogene Proteins B-raf
- Reproducibility of Results