Article
Development and Clinical Validation of Discriminatory Multitarget Digital Droplet PCR Assays for the Detection of Hot Spot KRAS and NRAS Mutations in Cell-Free DNA.
The Journal of molecular diagnostics : JMD - 1 Jul 2020
Hussung Saskia, Follo Marie, Klar Rhena F U, Michalczyk Sandra, Fritsch Kornelia, Nollmann Friederike, Hipp Julian, Duyster Justus, Scherer Florian, von Bubnoff Nikolas, Boerries Melanie, Wittel Uwe, Fritsch Ralph M
Abstract excerpt
Detection and quantification of tumor-derived KRAS and NRAS mutations in plasma cell-free DNA (cfDNA) holds great potential for cancer diagnostics and treatment response monitoring. Because of high sensitivity, specificity, robustness, and affordability, digital droplet PCR (ddPCR) is ideally suited for this application but requires discriminatory multiplexing when used as screening assay. We therefore designed,...
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